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Table 3 Interacting proteins that have both primary and secondary functions of moonlighting proteins in the MPR1-3 set

From: Genome-scale identification and characterization of moonlighting proteins

Moonlighting proteins

Interacting proteins

Uniprot ID/Name a)

Primary function b)

Secondary function c)

UniProt ID/Name d)

Interacting protein function e)

MP/non-MP f)

Ref.

P93834/HXK2

Glucose metabolism

Glucose signalling

Q42525/HXK1

1. Glycolysis

MP, I

[84]-[86]

2. Sugar mediated signaling programmed cell death

Q99798/ACO2

TCA cycle enzyme

Iron homeostasis

P21399/ACO1

1. Role in TCA cycle

MP, I

[87],[88]

mRNA binding and role in iron homeostasis

P00924/ENO1

Galactose catabolism enzyme

Homotypic vacuole fusion

P00925/ENO2

1. Glycolysis

MP, I

[89]

Vacuole fusion

Q08438/Vhs3

Halotorance determinant

Coenzyme A biosynthesis

P36024/SIS2

1. CoA biosynthesis

MP, I

[90]

Salt tolerance

P28482/ERK2

MAP kinase

Transcriptional

P01100

1. Regulation of transcription

MP, II

[91],[92]

  

Repressor

/FOS

2. Activates phospholipid synthesis in growing cells (regulated by Mos/MAP kinase pathway)

  

Q15796/SMAD2-5

1. TGF signaling protein

Multi-domain

[93]

2. Tumor suppressor, dual role in transcriptional activation

P05771/PRKCB

1. Serine/threonine-protein kinase, activates transcription.

MP, II

[94]

Inhibition of the insulin gene transcription.

O43293

1. Serine/threonine kinase

MP, I

[95],[96]

/DAPK3

2. Role in apoptosis, transcription, regulation of cell polarity, contractile processes in non-muscle or smooth muscle cells, and cytokinesis

P14921/ETS1

Transcription factor

nonMP

-

P19838/NFKB1

1. Transcription factor

MP, I

[97]

2. Cytoplasmic retention of attached NF-kappa-B proteins by p105, generation of p50 by a co-translational processing, transcriptional repressor

O43318

1. MAPK

MP, II

[98],[99]

/MAP3K7

Regulates TF activator proteins

Q99ML3/STAT3

Transcription factor

Electron transport chain

Q5EG47/Prkaa1-2

1. Protein kinase that phosphorylates TF

MP, II

[100]

Regulation of cellular energy

Q62120/Jak2-3

1. Tyrosine protein kinase

Multi-domain

[101]

Regulation of cellular signaling and cell cycle control

P05480/Src

1. Tyrosine protein kinase

Multi-domain

[102]

Cytokine/cellular receptor

O14786/Neuropilin-1

Vascular endothelial growth factor

Receptor for semaphorin III

Q14563/SEMA3A-G

1. Development of the olfactory system and in neuronal control of puberty

MP, II

[103]

2. Ensures proper endothelial abundance of soluble and alternatively spliced form of VEGF receptor(flt1)

P15692/VEGFA

Vascular endothelial growth factor

nonMP

-

P17948

1. VEGF receptor; plays negative role in angiogenesis in the embryo most likely by trapping VEGF

MP, I

[104]

/FLT1/VEGFR/

2. Plays positive role in adulthood in a tyrosine kinase-dependent manner

P54279/PMS2

Mismatch repair enzyme

Hypermutation of antibody variable chains

Q9JK91/Mlh1

1. Mismatch repair protein

MP, I

[105]

Somatic hyper mutation

P19971/PD-ECGF

Thymidine phosphorylase

Platelet-derived endothelial cell growth factor

P04183/TK1-2

1. Phosphotransferase activity

nonMP

-

Q96B60

Deoxyribonucleotidase, mitochondrial

nonMP

-

/NT5E, NT5M

P06744/Neuroleukin

Phosphoglucose Isomerase

Differentiation, maturation mediator

P52789/HK2

1. Hexokinase-2

MP, III

[106]

HK2 detachment causes apoptosis

P04075/ALDOA-C

1. Glycolysis and gluconeogenesis

MP, III

[107],[108]

Regulation of cell shape

P30613/PKLR

1. Pyruvate kinase

MP, III

[109]

Mutation causes hemolytic anemia

P14618/PKM2

1. Pyruvate kinase

MP, III

[110]

Programmed cell death

P06795/P-glycoprotein

P-glycoprotein (transporter)

Regulator of cell-swelling ion channel (K+/Cl-)

P41233/Abca1

Anion transporter

nonMP

-

Q91XR9/Phospholipid hydroperoxide glutathione peroxidase

Antioxidant of mature sperm

Structural protein of the mitochondrial capsule

Q60928/Ggt1

1. Part of the cell antioxidant defense mechanism

MP, IV

[111],[112]

2. Indirectly regulates multiple aspects of skeletal biology

Q9P2J5/Leucine-tRNA ligase

tRNA synthetases

Translocation and activation of mTORC1 to lysosomal membrane

Q9H6Q3/MARS

Methionine-tRNA ligase, cytoplasmic

nonMP

-

Q6P0M4/IARS

tRNA aminoacylation for protein translation

nonMP

-

P11325/Nam2p

Mitochondrial leucyl-tRNA synthetase

bI4 mitochondrial RNA splicing activity

P26637/CDC60

Leucine-tRNA ligase, cytoplasmic

nonMP

-

P19414/ACO1

TCA cycle enzyme

Mitochondrial DNA stability

P33421/SDH3

Succinate dehydrogenase involved in mt-electron transport chain

nonMP

-

  1. This table corresponds to Figure 6C.
  2. a)The name and UniProt ID of the moonlighting proteins in the MPR1-3 set.
  3. b)Primary function and c) secondary function of the moonlighting protein.
  4. d)The name and the UniProt ID of interacting proteins of the moonlighting protein shown in the left column.
  5. e)Multiple functions (if any) of the interacting protein.
  6. f)This column indicates if the interacting protein is a moonlighting protein (MP), not (non-MP), or multi-domain multi-functional protein (Multi-domain). The roman numerals, I to III, indicate the category of moonlighting proteins (see Table 1 caption).