Table 1 Procedures used in Urzymology
Process | Remarks |
|---|---|
Superimpose homologs | POSA server: http://fatcat.burnham.org/POSA/ |
ID shared invariant subset | Usually 10-30% of monomeric forms |
Re-design exposed surface | Protein design: Rosetta http://www.rosettacommons.org/ |
Clone & Express | Maltose Binding Protein (MBP) fusions; TEV cleavage |
Characterize | Rate accelerations, substrate specificities |
Authenticate | Single turnover active-site titration, Steady-state kinetics, genetic mutation and manipulation |
Evolutionary recapitulation | Multi-scale modular de- and reconstruction |