Thermostability measures in prokaryotic thermophile-mesophile ortologous protein datasets. (A-E) Receiver operating characteristic (ROC) curves for (hyper-)thermophile-mesophile homologous protein pairs from (A) one self-compiled dataset (Thermus thermophilus vs. Deinococcus radiodurans) and (B-E) four published datasets were tested with the different thermostability measures to discriminate thermophiles from mesophiles. Dataset 1 (B) is from ref. , dataset 2 (C) is from ref. , dataset 3 and 4 (D, E) are from ref.  (containing hyperthermophiles versus mesophiles and thermophiles versus mesophiles respectively).