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Figure 6 | Biology Direct

Figure 6

From: RNaseIII and T4 Polynucleotide Kinase sequence biases and solutions during RNA-seq library construction

Figure 6

Mapping patterns of the three whole transcriptome libraries. (a) 5’ read mapping results of the three whole transcriptome libraries were visualized at IGV. Ctl refers to the library constructed using RNaseIII. Heat refers to the library constructed using the heat fragmentation method. Heat + OptiK refers to the library constructed using the modified heat fragmentation method. The pile-ups of duplicated reads are indicators for fragment end sequence bias. The red circles represent the pile-ups. Ctl showed pile-ups. Heat had fewer numbers of pile-ups, and no pile-ups were found at Heat + OptiK. (b) 3’ read mapping results show the same patterns as the 5’ read mapping.

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