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Figure 2 | Biology Direct

Figure 2

From: RNaseIII and T4 Polynucleotide Kinase sequence biases and solutions during RNA-seq library construction

Figure 2

Mapping patterns of the gene specific library and the whole transcriptome library. (a) Mapped reads for the gene specific library were visualized at GABAB1 using IGV. (b) For the same genomic region as in (a), mapped reads for the whole transcriptome library were visualized. Compared to the gene specific library, the whole transcriptome library showed read pile-ups as well as gaps. At the gaps, no read was mapped at a known exon even though the gene specific library reads were mapped to these sites. In each figure, the top blue part shows the coverage of mapped reads, the middle grey part shows for individual mapped reads, and the bottom shows chromosome sequences and gene structures.

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