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Figure 10 | Biology Direct

Figure 10

From: RNaseIII and T4 Polynucleotide Kinase sequence biases and solutions during RNA-seq library construction

Figure 10

RNaseIII specific cutting sites. (a) Sequence biases of RNaseIII were summarized. RNaseIII specific cutting sites were mostly 5' - AA CC - 3'. (The 3' end reads are shown as reverse complement.) The red circle represents the 5' end cutting sites, and the blue circle represents the 3' end cutting site. (b) The figure shows how these cutting sites map onto the hairpin regions cut by RNaseIII. The sites are disconnected by short fragment RNAs. The RNaseIII specific cutting sites of our data are at the long fragment ends.

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