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Figure 1 | Biology Direct

Figure 1

From: RNaseIII and T4 Polynucleotide Kinase sequence biases and solutions during RNA-seq library construction

Figure 1

Preparation of gene specific library. mRNA is visualized as a grey box flanked by black lines. The grey box represents the open reading frame (ORF), and the black lines represent UTRs. We obtained total RNA and depleted rRNA from it. Reverse transcription was carried out using a GABAB1 gene specific primer. The resulting single stranded complementary DNAs (sscDNAs) were converted to dscDNAs via template switching. After amplification with another gene specific primer, dscDNAs were sonicated. Adaptor ligated dscDNAs were sequenced with the SOLiD system.

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