TY - JOUR AU - Lee, Changhoon AU - Harris, R. Adron AU - Wall, Jason K. AU - Mayfield, R. Dayne AU - Wilke, Claus O. PY - 2013 DA - 2013/07/04 TI - RNaseIII and T4 Polynucleotide Kinase sequence biases and solutions during RNA-seq library construction JO - Biology Direct SP - 16 VL - 8 IS - 1 AB - RNA-seq is a next generation sequencing method with a wide range of applications including single nucleotide polymorphism (SNP) detection, splice junction identification, and gene expression level measurement. However, the RNA-seq sequence data can be biased during library constructions resulting in incorrect data for SNP, splice junction, and gene expression studies. Here, we developed new library preparation methods to limit such biases. SN - 1745-6150 UR - https://doi.org/10.1186/1745-6150-8-16 DO - 10.1186/1745-6150-8-16 ID - Lee2013 ER -