Figure 4

Hypermethylation of C14 in GBM. R1 to R10 represent 10 random regions of the genome having similar number of methylation probes as that of C14 (~200 probes). We have used another imprinted microRNA region, present on Chr19, as a control (C19) to test the specificity of the methylation pattern on C14 in GBM. The relative methylation levels are plotted along the vertical axis (beta values). The jitter plots in the background represent the total distribution of the beta-values in each region for all samples. The box plots determine the median beta-values of those regions. C14 is found to be hypermethylated having median beta-value >0.8 (p<0.003), whereas the other sets, including C19 are random in distribution within the normal range (0.2 to 0.8).