Figure 3

Structural analysis of the DUF71 (PF0828) putative activesite. (A) Docking of modified EF-2 (cyan, PDB id: 3B82) onto ATP-bound structure of PF0828 (yellow, PDB id: 3RK1). ATP and several residues of PF0828 (DUF71), which are conserved among archaeal and eukaryotic orthologs, and diphthine of EF-2 (see text for details) are shown in stick models. (B) Close-up stereo pair of panel A. Diphthine of EF-2 and the side chains of conserved residues of PF0828, at the interface of PF0828 and EF-2, are shown in stick models and labeled. (C) Stereo pair view of ATP-binding region of PF0828. Residues that are conserved among Dph6 and DUF71-B12 families are depicted in stick models with carbon atoms in cyan, while the residues that are specific to Dph6 family are shown in stick models with carbon atoms in green. Oxygen and nitrogen atoms are shown in red and blue in all stick models, respectively.