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Figure 3 | Biology Direct

Figure 3

From: CRISPR transcript processing: a mechanism for generating a large number of small interfering RNAs

Figure 3

Kinetics of crRNA accumulation. The figure shows how pre-crRNA (the first row) and crRNA (the second row) amounts change as pre-crRNA processing rate (k) is increased. CRISPR transcription rate remains constant and has the same value as in Figure 2. The first and the second column correspond, respectively, to A) equilibrium transcript amounts and B) transcript amounts at 20 min post-induction. The horizontal axes in the figure correspond to k in multiples of λu, where k changes from the uninduced value (λu/100) to a very high value (1000λu). The points on the horizontal axes are, for clearer presentation, plotted equidistantly, and correspond to k (in multiples of λu) values of: (1/100, 1/50, 1/10, 1, 10, 50, 100, 500, 1000). The magenta line and the blue triangles correspond, respectively, to the stochastic and the deterministic simulations. The figure confirms the saturation effects observed in Figure 3, and suggests that the system is able to generate substantial crRNA amounts soon after its induction.

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