Figure 4

Rolling circle replicase (Rep) amino acid multiple sequence alignment. The BSL RDHV Rep ORF sequence (BSL_Rep_ORF) is aligned to closely related sequences retrieved from NCBI using PSI-BLAST searches. Sequence alignment was performed using ClustalW set to default parameters in MEGA v.5. The endonuclease domain motifs I, II and III, and superfamily-3 helicase domain Walker A P-loop NTPase (W-A P-loop), Walker B (W-B), B’ and C motifs are boxed and labeled. Rep nuclease (RCRE) and superfamily-3 helicase (S3H helicase) domains are indicated by block arrows above the sequence alignment. A ClustalW color scheme is applied to the multiple alignment using Jalview. The range of amino acid residues used in the alignment are shown after the sequence name. (GOS) Global Ocean Survey paired-end read scaffolds 10666 and 6801. CIRCOVIRIDAE: (PCV2) Porcine Circovirus-2, (StCV) Starling Circovirus, (BatCV) Bat Circovirus, (DfCyV) Dragonfly Cyclovirus. NANOVIRIDAE: (BBTV) Banana Bunchy Top Virus, (SCSV) Subterranean Clover Stunt Virus.