Evaluation of antigen-specific and non-specific immunoglobulin responses. PsaA specific serum antibody responses were determined for BALB/c mice immunized with the indicated recombinant proteins. Mice were immunized on weeks 0 and 3 and bled at weeks 2 and 5 for evaluation of primary (A, C and E) and secondary (B, D and F) antibody responses to PsaA, human BLyS and dsDNA, respectively, as indicated. Serial dilutions of individual sera from 5 mice were tested for total (Igκ) antibodies to specific antigens by ELISA and are plotted as means ± SEM for 5 mice per group. G, Mice immunized twice with the indicated antigens as in Figure 2B were boosted with PsaA alone and bled at week 9 for PsaA specific antibody responses. H, The relative immunogenicities of PsaA administered with various adjuvant formulations was tested in C3H/HeJ mice. Groups of mice were immunized once and bled after 2 weeks for the evaluation of PsaA-specific total antibody responses by ELISA. Data are presented as means ± SEM for 5 mice per group.