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Figure 1 | Biology Direct

Figure 1

From: Impact of Alu repeats on the evolution of human p53 binding sites

Figure 1

Four p53 core domains bound to bent DNA. It is based on the computational model [45] that was further corroborated by gel electrophoresis experiments [46]; the overall DNA bend is ~40°. The spacer S between two decamers varies from 0 to 18 bp in the known functional p53 REs [5, 6]. The shown structure corresponds to S = 0. The red arrows show the major-groove bending (M) in the CWWG tetramers; blue arrow is for the minor-groove bend (m) in the center of the site. The lateral positioning of p53 DBDs on the external side of DNA loop and the degree of DNA bending imply that in principle, the p53 tetramer can bind to nucleosomal DNA. The dashed lines indicate that the N-termini N1-N4 are accessible for interactions with trans-activation and trans-repression factors (e.g., histone acetylase, HAT, and histone deacetylase, HDAC). Large colored arrows indicate the orientations of the four p53 subunits.

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