Figure 2
From: Functional analysis of archaeal MBF1 by complementation studies in yeast
Functional complementation of yeast mbf1 Δ by archaeal MBF1 from T. tenax (T) and M. mazei (M). (A) AT sensitivity of yeast wild type (WT), the mbf1Δ strain carrying pyMBF1 (+), empty vector (-), pTMBF1 (TTT) and pMMBF1 (MMM) using the vector pRS316 under control of the natural yeast MBF1 regulatory region. Serial dilutions of the respective strains were placed on minimal medium either in the presence (+AT) or absence (-AT) of 3 mM aminotriazole. The plates were incubated at 30°C for 3 days. (B) Expression of TMBF1 protein (pHRTMBF1) in the ymbf1Δ strain was detected by immuno blotting using a polyclonal antibody against TMBF1.