Undetected antisense tRNAs in mitochondrial genomes?

Biology Direct

Table 5 Correlations of genome-wide amino acid usages with misacylation potential, cloverleaf stability and processing inaccuracy of antisense tRNAs predicted to be loaded by that amino acid.

tRNA		TFAM		COVE		resCOVE		Spacer
Processed	n	r	rs	r	rs	r	rs	r	rs
Ala	39	-0.29	-0.32	0.29	0.38	0.28	0.35	-0.37	-0.47
Asp	44	0.01	0.02	0.23	0.23	0.23	0.23	-0.15	-0.12
Gln	36	-0.30	-0.46	0.50	0.52	0.51	0.55	-0.02	-0.11
Glu	44	-0.14	-0.25	-0.08	-0.05	-0.12	-0.11	-0.10	-0.10
Ile	48	0.02	-0.04	0.04	0.09	-0.13	-0.05	0.04	0.01
Met	49	0.09	-0.16	-0.16	-0.21	-0.14	-0.12	-0.16	-0.14
Pro	43	-0.26	-0.28	0.04	0.12	0.01	0.13	0.10	0.11
Ser UCN	29	0.26	0.19	0.18	0.23	0.17	0.21	-0.19	-0.20
Thr	34	0.24	0.21	-0.11	-0.04	-0.09	-0.17	0.04	0.22
Trp	49	-0.19	0.17	-0.52	-0.56	-0.53	-0.54	0.04	0.16
Tyr	37	-0.04	-0.22	-0.39	-0.41	-0.37	-0.40	0.05	0.13
Unprocessed
Arg	49	0.17	0.20	0.14	0.17	-0.10	-0.04
Asn	49	0.22	0.17	0.06	0.10	0.11	0.14
Cys	48	0.31	0.29	0.34	0.33	0.33	0.35
Gly	46	-0.05	0.02	0.13	0.08	0.14	0.13
His	48	0.01	0.02	0.29	0.24	0.30	0.25
Lys	20	0.11	0.07	0.42	0.39	0.38	0.30
Phe	44	-0.24	-0.13	0.33	0.34	0.29	0.32
Val	46	0.05	-0.01	0.05	-0.02	0.07	-0.03
Leu CUN	6	0.29	-0.22	-0.69	-0.73	-0.65	-0.64
Ser AGY	49	0.43	0.40	0.33	0.24	0.33	0.24
% as expected		36	46	68	64	59	55	55	55

The extension hypothesis expects negative correlations for misacylation and processing inaccuracy, and positive ones between cloverleaf stability (COVE) and amino acid usage. The last row indicates the percentage of correlations whose directions match predictions.

ISSN: 1745-6150