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Table 1 Anticodons of sense and antisense tRNAs in vertebrate mitochondria for heavy- and light-strand encoded sense tRNAs and cognate amino acid matching sense and antisense anticodons according to the vertebrate mitochondrial genetic code.

From: Undetected antisense tRNAs in mitochondrial genomes?

Sense tRNA

  

Antisense tRNA

    

Cognate

Anticodon

COVE

Anticodon

Cognate

COVE

Processing

Dist.

Heavy strand

       

Ala

TGC

23.03

GCA, undet

Cys, ?

1.9

3'

8

Asn

GTT

18.2

AAC, undet

Val, ?

12.04

none

 

Cys

GCA

20.27

TGC

Ala

-7.83

none

 

Gln

TTG

18.53

CAA

Leu UUR

-20.71

5', 3'

4

Glu

TTC

22.46

GAA

Phe

3.56

5'

5

Pro

TGG

30.29

CCA

Trp

16.31

3'

2

Ser UCN

TGA

11.06

TCA

Trp

12.5

5', 3'

2

Tyr

GTA

16.92

TAC

Val

-1.55

5'

13

Light strand

       

Arg

TCG

28.22

CGA

Ser UCN

21.2

none

 

Asp

GTC

37.53

GAC

Val

15.15

3'

2

Gly

TCC

37.42

GGA

Ser UCN

18.42

none

 

His

GTG

21.64

CAC

Val

16.99

none

 

Ile

GAT

29.27

ATC

Asp

10.84

5'

2

Leu UUR

TAA

21.47

TTA, undet

Stop, ?

-15.65

none

 

Leu CUN

TAG

30.67

CTA, TAA

Stop, Leu UUR

3.56

none

 

Lys

TTT

33.39

AAA, undet

Phe, ?

21.53

none

 

Met

CAT

12.41

ATG

His

11.71

3'

2

Phe

GAA

17.72

TTC

Glu

4.61

none

 

Ser AGY

GCT

 

AGC, undet

Ala, ?

4.75

none

 

Thr

TGT

28.23

ACA

Cys

9.87

5'

2

Trp

TCA

31.2

TGA

Ser UCN

3.35

5'

8

Val

TAC

21.88

GTA

Tyr

6.78

none

 
  1. The 3d and 6th columns indicate COVE scores for the secondary structure component of the tRNA's cloverleaf, calculated by tRNAscan-SE (http://lowelab.ucsc.edu/tRNAscan-SE/[37]). The 4th column indicates the antisense anticodon as the inverse complement of the sense anticodon in column 2. If tRNAscan-SE detected a different (or no) anticodon, this is indicated after the comma. The 5th column indicates the amino acid matching the antisense anticodon according to the vertebrate mitochondrial genetic code. Column 7 indicates whether normal mitochondrial RNA maturation processes the 5' and/or 3' extremities of the antisense tRNA. The last column indicates the distance in number of nucleotides between these extremities of the antisense tRNA and the sense gene processed by regular sense RNA maturation