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Figure 1 | Biology Direct

Figure 1

From: Novel eukaryotic enzymes modifying cell-surface biopolymers

Figure 1

Multiple alignment of representatives of the PC-Esterase family. Multiple sequence alignment of the PC-Esterase domain was constructed using Kalign after parsing high-scoring pairs from PSI-BLAST search results. The alignment was refined based on the pairwise alignments produced by the profile-profile searches with the HHpred program using the PC-esterase profile. The secondary structure from the crystal structures is shown above the alignment with E representing a strand and H a helix. The 85% consensus shown below the alignment was derived using the following amino acid classes: hydrophobic (h: ALICVMYFW, yellow shading); small (s: ACDGNPSTV, green); polar (p: CDEHKNQRST, blue) and its charged subset (c: DEHKR, pink), and big (b: FILMQRWYEK; grey shading). The limits of the domains are indicated by the residue positions, on each end of the sequence. The numbers within the alignment are non-conserved inserts that have not been shown. The sequences are denoted by their gene name followed by the species abbreviation and GenBank Identifier (gi). The species abbreviations are Afum: Aspergillus fumigatus; Atha: Arabidopsis thaliana; Bflo: Branchiostoma floridae; Bfuc: Botryotinia fuckeliana; Caps: Capitella spI; Cele: Caenorhabditis elegans; Cneo: Cryptococcus neoformans; Dpul: Daphnia pulex; Efae: Enterococcus faecalis; Hsap: Homo sapiens; Lgig: Lottia gigantea; Mmus: Mus musculus; Nvec: Nematostella vectensis; Ppat: Physcomitrella patens; Spur: Strongylocentrotus purpuratus; Xtro: Xenopus tropicalis. The bacterial (1yzf) prototype of the GDSL/SGNH family is the first sequence shown. The conserved residues of this family are marked by triangles above the alignment, with the red triangles denoting active residues. The conserved residues of the PC-Esterase domains are shown using circles below the alignment, with the red ones denoting active residues. The PC-Esterase domains also have an extra N terminal Helix-0 that is not seen in the canonical SGNH domains. The PC-Esterase domain in the Lottia gigantea protein has a NxxxH instead of a DxxH, where the polar N has shifted one amino acid to the left. The metazoan C7orf58 have a S in place of the D in the catalytic triad, but the hydroxyl group of this residue can function equivalently. The subfamily names are shown to the right.

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