Skip to main content
Figure 5 | Biology Direct

Figure 5

From: CD44 expression positively correlates with Foxp3 expression and suppressive function of CD4+ Treg cells

Figure 5

Regulatory function of CD4+ CD25+ CD44+ and CD4+ CD25+ CD44- splenocytes. (A) CD4+CD25+CD44+ and CD4+CD25+CD44-cells were sorted from the splenocytes of C57BL/6 mice, and these cells (1 × 105/well) were co-cultured with naïve T cells (2 × 105/well) in the presence of Con A for 72 h. T cell proliferation was measured by incorporation of [3H]-thymidine. Data are presented as counts per min, and shown are the mean ± S.D. from three independent experiments. (B) Total RNA was extracted from purified CD4+CD25+CD44+ and CD4+CD25+CD44- cells for RT-PCR analysis of IL-10 expression. (C) The integrated density values (IDV) for the IL-10 transcripts were quantitated and normalized to those of β-actin. Shown are representative results from one of three independent experiments. (D) The data were pooled from three independent experiments and shown in the plot. (E) The levels of IL-10 mRNA among different subsets of CD44+ expressing cells. CD4+CD25+CD44high, CD4+CD25+CD44med and CD4+CD25+CD44low cells were sorted from the spleens of naïve mice. (F) CD4+CD8-CD25+CD44+, CD4+CD8-CD25+CD44-, CD4+CD8-CD25-CD44+, and CD4+CD8-CD25+CD44- cells were sorted from the thymus of naive mice. Total RNA was isolated for measuring IL-10 mRNA by real-time RT-PCR. Data are presented as fold-induction relative to the levels of β-actin. Shown are representative results one of three independent experiments.(* p < 0.05, ** p < 0.01, *** p < 0.001).

Back to article page