Figure 3

Foxp3 expression in different subsets of thymocytes. Thymocytes were stained with antibodies against CD4 (PE-Cy7), CD8 (FITC), CD44 (PE), CD25 (APC-Alexa Fluor755), and Foxp3 (PE-Cy5). CD4^-CD8^- DN, CD4⁺CD8^- SP, CD4^-CD8⁺ SP, and CD4⁺CD8⁺ DP thymocytes were gated for subsequent analyses. (A) Foxp3 expression (%) in CD4⁺CD25⁺ cells that was either positive or negative for CD44. (B) Foxp3 expression (%) in CD4⁺CD25⁺ cells that expressed high, medium, or low levels of CD44. (C) Foxp3 expression (%) in CD4⁺ cells that was either positive or negative for CD25. (D) Foxp3 expression (%) in CD4⁺ cells that expressed high, medium, or low levels of CD44. (E) CD44 expression (%) in CD4⁺ cells that was either positive or negative for Foxp3. Panels A to E show representative results with mean ± S.D. from three independent experiments (* p < 0.05, ** p < 0.01, *** p < 0.001).