Figure 2

TCR-β expression on CD4⁺ CD8^- CD25⁺ CD44⁺ T _reg cells in mouse thymus. Thymocytes were collected from naïve mice, stained with antibodies against CD4 (PE-Cy7), CD8 (FITC), CD44 (PE), CD25 (APC-Alexa Fluor755), and TCR-β(PerCp), and analyzed by flow cytometry. (A) CD4^-CD8^- (P4), CD4⁺CD8⁺ (P3), and CD4⁺CD8^- cells (P5) were gated from total, live thymocytes (P1). Cells from each of the quadrants (designated as P3-P5) were subgated into CD25⁺CD44^- and CD25⁺CD44⁺ (In Figure 2A, - and + mean CD25⁺CD44^- and CD25⁺CD44⁺ cells [analysis described in panel B]). In addition, CD4⁺CD8^- SP cells were subgated into CD25⁺CD44^low, CD25⁺CD44^med, and CD25⁺CD44^high cells (analysis described in panel C). (B) The percentages of TCR-β cells in CD4⁺CD8^- SP cells (mean ± S.D., n = 3). (C) The percentages of TCR-β cells among CD4⁺CD25⁺CD44^low, CD4⁺CD25⁺CD44^med, and CD4⁺CD25⁺CD44^high cells. Statistical significance in panels B and C is indicated by * p < 0.05 and ** p < 0.01.