Tumor growth of maGSC Stra8 SSC5 cells after injection into immunodeficient SCID/beige mice. Undifferentiated maGSC Stra8 SSC5 cells or in vitro differentiated cells (5 + 10 days) were injected subcutaneously at day 0 into SCID/beige mice (2 × 106 cells per mouse). The undifferentiated cells were cultured under the four conditions (I to IV) before the injection. The tumor size was recorded every second day until day 100 using linear calipers. (A) The growth of tumors in individual mice is shown. The mice were injected with cells from two to three independent cultures that are indicated by different colors. Animals were sacrificed after day 100 or before when ethical criteria for the stop of the experiment were reached. (B) The same data are presented on a different scale for the tumor size in order to show small or regressing tumors.