Figure 2

Phylogenetic relationship and domain architectures of MAEL proteins. (A) An unrooted phylogenetic tree was reconstructed using maximum likelihood (ML) analysis and Bayesian analysis. Single MAEL domains are represented by species names. The duplicated ones in the species of Aa, Ci, Cp, Ed, Eh are represented by species names following Genbank ID, whereas Cs domains are represented by Cs1 and Cs2. Branch length is proportional to estimated evolutionary change by PhyML program; the scale bar represents 0.5 substitution per site. Node supporting values greater than 75% from ML bootstrap analyses and Bayesian MCMCMC sampling are shown on the left and on the right of the slash, respectively. Lineage-specific expansions of MAEL domains in amoeba, mosquito and sea squirt are highlighted with different colors (red, turquoise blue and pink) and ancient duplication events were indicated by circled numbers. The loss of MAEL in teleost fish is indicated in blue dashed. Asterisk labeled MAEL domains are the ones containing both conserved EHHCHC and EDDHD residues (see following main text). (B) Domain architectures of representatives of the MAEL proteins were deduced through searching against Pfam and SMART domain databases and drawn approximately to scale. The domains shown are: HDAC_interact, named after Histone deacetylase (HDAC) interacting (SMART: SM00761); HMG, named after High Mobility Group (SMART: SM00398); SR-25-like (DUF1777, Pfam: PF08648). New species name abbreviations: Bm, Brugia malayi; Bt, Bos taurus; Cf, Canis familiaris; Cp, Culex pipiens quinquefasciatus; Dp, Drosophila pseudoobscura; Dy, Drosophila yakuba; Ec, Equus caballus; Lb, Leishmania braziliensis; Ll, Lutzomyia longipalpis; Mm, Mus musculus; Mu, Macaca mulatta; Nv, Nasonia vitripennis; Rn, Rattus norvegicus; Sp, Strongylocentrotus purpuratus; Ss, Sus scrofa; Tc, Tribolium castaneum. Other species name abbreviations refer to Figure 1 legend.