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Figure 1 | Biology Direct

Figure 1

From: Unraveling the biochemistry and provenance of pupylation: a prokaryotic analog of ubiquitination

Figure 1

PafA family gene neighborhoods, PafA topology diagram, and reactions catalyzed by PafA and related enzymes. Conserved gene neighborhoods in (A) are depicted as arrows with the arrowhead pointing the 5' to 3' direction. The neighborhoods are labeled below with the name of a representative organism and the gene name corresponding to the PafA gene(s) from that neighborhood. The topology diagram in (B) depicts idealized conserved core secondary structural elements of the family. Elements that can be highly variable in the entire GS fold are shaded in gray. Conserved elements are labeled in the order in which they appear in the structure. Conserved residues contributing to catalysis are rendered as line drawings. The known and predicted reaction schemes for different members of the carboxylate-amine ligase superfamily are shown in (C). The member catalyzing the reaction is listed to the left of the reaction. In the case of GatB/GatE the reaction is catalyzed in situ on tRNA charged with a glutamate residue.

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