Sequence conservation in the GFP family. Multiple alignment of protein sequences of GFP-like proteins from cnidarians, copepods, and lancelet. Protein sequences of gene products predicted from the genome assembly of B. floridae were clustered at the 90% identity cutoff, and one representative per cluster that did not contain internal deletions was included into the alignment (see Table 1 for details). Identifier of each sequence in JGI genome browser or in GenBank is given after each sequence. The consensus secondary structure derived from multiple known three-dimensional structures of GFP-like proteins is shown below the alignment. Red type indicates conserved small or kinky side chains (G, S, A, or P), yellow shading indicates conserved bulky hydrophobic residues (I, L, V, M, F, Y, or W), blue type indicates conserved acidic or amidic residues (D, E, N, or Q), blue shading indicates conserved basic residues (K or R), purple type with gray shading indicates the tripeptide directly participating in rearrangement that leads to the chromophore formation, and white type on black indicates the amino acid whose codon contains an intron in the known genome sequence. Species abbreviations are as follows: Aeqvi, Aequorea victoria; Astla, Astrangia lajollaensis; Chipo, Chiridius poppei; Corca, Corynactis californica; Dissp, Discosoma sp. RC-2004; Monca, Montastraea cavernosa; Monef, Montipora efflorescens; Nemve, Nematostella vectensis; Phial, Phialidium sp. SL-2003; Ponpe, Pontella meadi; Ponpl, Pontellina plumata; Renmu, Renilla muelleri.