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Table 1 Details regarding the samples include bird identification and species, isolate of P. relictum and island of origin of isolate; details regarding the methods include enzyme/buffer system and number of cycles for amplification, and method of amplification and cloning*

From: Genetic characterization of Hawaiian isolates of Plasmodium relictumreveals mixed-genotype infections

Bird (DNA log)

Species

Isolate

Tissue Collection Date

Island

Enzyme

Number of cycles

Method

Total clones

Confirmed RFLP variants

Confirmed RFLP clones

Number of sequences

1 (764)

Hemignathus virens

K1

Survivor Day 89 PI

Kauai

Promega

80

PCR+1

20

4

16

0

2 (1145)

Hemignathus virens

K1

Survivor Day 81 PI

Kauai

Promega

80

PCR+1

20

6

16

1

3 (1187)

Hemignathus virens

K1

Survivor Day 69 PI

Kauai

Promega

80

PCR+1

20

5

17

0

4 (584)

Hemignathus virens

K1

Fatality Day 16 PI

Kauai

Promega

80

standard

17

4

15

1

     

Promega

80

PCR+1

20

5

18

1

5 (591)

Hemignathus virens

K1

Fatality Day 19 PI

Kauai

Promega

80

standard

9

4

9

0

     

Promega

80

PCR+1

20

5

17

1

6 (593)

Hemignathus virens

K1

Fatality Day 19 PI

Kauai

Promega

80

standard

10

2

5

0

     

Promega

80

PCR+1

20

5

16

0

     

Promega

35

PCR+1

19

3

15

0

     

Roche

35

PCR+1

20

2

20

10

7 (601)

Hemignathus virens

KV115

Fatality Day 16 PI

Hawaii

Promega

80

PCR+1

20

5

16

1

8 (613)

Hemignathus virens

KV115

Fatality Day 23 PI

Hawaii

Promega

80

standard

9

5

8

0

9 (736)

Hemignathus virens

KV115

Survivor Day 81 PI

Hawaii

Promega

80

PCR+1

20

8

17

4

10 (620)

Hemignathus virens

KV115

Fatality Day 24 PI

Hawaii

Promega

80

standard

17

7

17

0

     

Promega

80

PCR+1

20

5

17

1

11 (623)

Hemignathus virens

KV115

Fatality Day 26 PI

Hawaii

Promega

80

standard

9

2

6

0

     

Promega

80

PCR+1

20

4

13

0

     

Promega

35

PCR+1

20

4

18

0

     

Roche

35

PCR+1

20

2

18

10

12 (1757)

Hemignathus virens

wild

Blood 4/20/96

Kauai

Promega

80

standard

8

3

6

1

13 (1765)

Vesteria coccinea

wild

Blood 4/5/95

Hawaii

Promega

80

standard

9

5

9

1

14 (1818)

Himatione sanguinea

wild

Blood 3/28/95

Hawaii

Promega

80

standard

10

5

9

0

15 (1928)

Hemignathus virens

wild

Blood 11/16/93

Maui

Promega

80

standard

3

1

2

0

16 (2881)

Chasiempis sandwichensis

wild

Blood 2/3/95

Kauai

Promega

80

standard

9

4

8

1

17 (3186)

Chasiempis sandwichensis

wild

Blood 3/13/96

Kauai

Promega

80

standard

7

6

6

1

18 (6775)

Hemignathus virens

wild

Blood 4/24/01

Hawaii

Promega

80

standard

1

1

1

1

Totals

       

397

 

335

33

  1. *Details regarding the clones include total number produced, number of confirmed (detected in > 1 bird) variants and clones, as well as the number of clones sequenced. Blood was collected from experimentally-infected birds that survived infection between days 69–89 post-infection (PI) when parasitemias had reached chronic levels. Pectoral muscle was collected from experimentally-infected birds that succumbed to infection between days 16–26 PI when acute phase parasitemias were high.