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Figure 4 | Biology Direct

Figure 4

From: 14-3-3 theta binding to cell cycle regulatory factors is enhanced by HIV-1 Vpr

Figure 4

Cell cycle regulatory protein binding to 14-3-3 θ is also enhanced during G2,M arrest induced by adriamycin. Jurkat T cells were treated with adriamycin (Adr; 0.2 μg/ml) for 24, 48, or 72 hours (h) or untreated (0) and examined for 14-3-3 θ co-immunoprecipitating (IP) proteins. (A) Western blot analysis of Plk1, CyclinB1, Cdk1, 14-3-3 θ and centrin in whole cell lysates before IP (input) and after 14-3-3 θ IP (IP: 14-3-3 θ) at the time of adriamycin treatment indicated. These data are representative of three independent experiments. Although the amount of 14-3-3 θ appears less in the untreated (0) time point of the IP, this was not a reproducible finding. (B) Viability of the samples used in the IP in (A) determined by flow cytometry detection of propidium iodide (PI) exclusion and large size (high forward scatter). The percentage of viable cells is indicated. Flow cytometric histograms of the DNA content for each sample determined by propidium iodide DNA staining (x-axis) are shown as an inset.

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