Figure 2

Examples of putative conserved introns. Sequences were selected that had splice site regions (color red) in which at least 6 out of 10 residues were similar (in bold). Of those, only sequences were selected where also at least 6 residues up- and downstream of the splice site matched (in black, matching residues in bold). This minimized false-positive conserved splice sites, although most sequences that had a similar splice site (> 5/10 similar residues) already showed homology in the rest of the sequences. All matching sequences are between distantly related eukaryotic kingdoms, e.g. between Protists, Fungi, Animals and Plants, ensuring that a match would have originated close to the root of the Eukaryotic Tree. For each set, its conserved domain as retrieved from CDD (shaded box) is shown, while the columns show the species name, the sequence around the splice site (splice site given by | symbol), the phase of the intron and a shortened gene annotation.