Figure 5

Natural tolerance of donor lymphocytes when present systemically but not when restricted to a peripheral site. Donor cell chimerism assessed from 50–150 days post B6 fetal liver immune reconstitution of RAG vs. RAG/γc mice previously injected i.v. vs. i.p. with donor BALB/c peritoneal cells (as described in Figure 4A). (A) Representative flow cytometry data assessing donor cell chimerism amongst recipient peritoneal cells. Donor and recipient cells are defined by the upper left and lower right box in each top row dot plot respectively. Lower dot plots show recipient B and T cell reconstitution after gating on host cells as shown in the lower right box of the upper dot plots. (B) The percentage of donor cells in the spleen and peritoneum of individual recipients. Values for spleen plus peritoneum comparing RAG/γc vs. RAG: i.v. p < 0.01, and i.p. p < 0.001.