Eukaryotic RNAi | Domains/function | CASS | Domains/function |
---|---|---|---|
Dicers | Helicase/RNAseIII. Processing of long dsRNA into siRNA and pre-miRNA into miRNA, involves unwinding | Helicase (COG1203) + HD nuclease (COG2254) - fused or adjacent genes, | SFII helicase + HD nuclease |
Argonautes/slicers | Ferredoxin-fold-PAZ-PIWI – endonuclease, target degradation | RecB-family nuclease (COG1468, 4343); COG1857 – a novel nuclease? | Target degradation |
R2D2/RDE-4 | dsRNA-binding domain, interacts with Dicer | RAMPs | Ferredoxin-fold duplication. Size-specific psiRNA-binding, pre-psiRNA-binding, other RNA-binding functions? |
Fmr1/Fxr | RGG, KH-ssRNA-binding | RAMPs | Ferredoxin-fold duplication. Size-specific psiRNA-binding, pre-psiRNA-binding, other RNA-binding functions? |
Tsn | Tudor, SN – RNA-binding | RAMPs | Ferredoxin-fold duplication. Size-specific psiRNA-binding, pre-psiRNA-binding, other RNA-binding functions? |
Vig | RGG – RNA-binding | RAMPs | Ferredoxin-fold duplication. Size-specific psiRNA-binding, pre-psiRNA-binding, other RNA-binding functions? |
RNA-dependent RNA polymerase | RdRp domain related to DdRp; 2nd-strand synthesis for siRNA production | Predicted RdRp/RT (COG1353) | Palm polymerase domain. 2nd strand synthesis for psiRNA production, reverse transcription for CRISPR formation |