Figure 9
Immunofluorescence localization of NMDA zeta receptor subunit (NR1), Hsc70, and synaptotagmin VII to epithelial tight junctions. (A) Confocal images showing immunofluorescence localization of postsynaptic NMDA receptor zeta subunit co-localized with occludin in MDCK I cells (one week post-confluent). (B) Confocal images showing immunofluorescence localization of presynaptic Hsc70 localized to tight junction domains of C2bbE1 cells (4 weeks post-confluent). (C) Confocal images showing immunofluorescence localization of presynaptic synaptotagmin VII localized to tight junction domains of C2bbE1 cells (4 weeks post-confluent). Note the punctate patterns of NMDA zeta, Hsc70, and synaptotagmin VII.