Table 1 Table of the mean coefficients of standard deviation characteristic function. The table shows the average values for parallel hybridization, parallel biological cultures and pooled reference sample. The coefficients a and b are the coefficients of the standard deviation function; CV stands for the coefficient of variation.
Comparison | a | b |
|---|---|---|
GM10469 Hybridization 1 vs hybridization 2 | Â | Â |
   Hybridization 1 vs. 2: C1a vs C1b | 2.97 | 0.048 |
   Hybridization 1 vs. 2: C2a vs C2b | 3.24 | 0.040 |
   Hybridization 1 vs. 2: C3a vs C3b | 4.27 | 0.034 |
   Hybridization 1 vs. 2: C4a vs C4b | 3.71 | 0.037 |
   Hybridization 1 vs. 2: C5a vs C5b | 3.54 | 0.074 |
Average (n = 5 pairs) | 3.54 | 0.047 |
Coefficient of variation | 0.14 | 0.35 |
Combined Series (C1a – C4a) vs (C1b-C4b) | 3.51 | 0.043 |
Biological replicates | Â | Â |
Series Ca, all pairwise hyb 1; C1a-C5a | Â | Â |
Average (n = 10) | 3.86 | 0.130 |
Coefficient of variation | 0.08 | 0.27 |
Series Ca, mean based on individual genes (n = 5) | 3.39 | 0.120 |
Series Cb, all pairwise hyb 2; C1b-C5b | Â | Â |
Average (n = 10) | 3.08 | 0.136 |
Coefficient of variation | 0.05 | 0.31 |
Series Cb, mean based on individual genes (n = 5) | 3.00 | 0.116 |
Hybridization variation | Â | Â |
   Pooled reference RNA (PR1a vs PR1b) | 2.12 | 0.045 |
   Pooled reference RNA (PR2a vs PR2b) | 3.52 | 0.082 |
   Pooled reference RNA (PR3a vs PR3b) | 4.86 | -0.003 |
   Pooled reference RNA, hybridization 1 vs 2 |  |  |
Average (n = 3 pairs) | 3.50 | 0.042 |
Coefficient of variation | 0.39 | 0.61 |
Transcription and hybridization variation | Â | Â |
   Series 1 |  |  |
   PR1a vs PR2a | 2.90 | 0.128 |
   PR1a vs PR3a | 3.30 | 0.130 |
   PR2a vs PR3a | 4.45 | 0.103 |
Average (n = 3 pairs) | 3.55 | 0.120 |
Coefficient of variation | 0.23 | 0.13 |
Series 2 | Â | Â |
   PR1b vs PR2b | 3.20 | 0.184 |
   PR1b vs PR3b | 3.33 | 0.118 |
   PR2b vs PR3b | 4.68 | 0.153 |
Average (n = 3 pairs) | 3.73 | 0.152 |
Coefficient of variation | 0.22 | 0.22 |