Fig. 6

TNK2 is essential for ZNF692-mediated activation of MEK/ERK signaling pathway. (A-C) Western blot and quantitative analyses of MEK1/2, P-MEK1/2, ERK1/2, and P-ERK1/2 in osteosarcoma cells transfected with siRNA specifically targeting TNK2 (siTNK2) and negative control (siNeg). (D-F) Western blot and quantitative analyses of MEK1/2, P-MEK1/2, ERK1/2, and P-ERK1/2 in osteosarcoma cells transfected with empty vector and TNK2 overexpression plasmid. (G-I) Western blot and quantitative analyses of MEK1/2, P-MEK1/2, ERK1/2, and P-ERK1/2 for the indicated groups revealed that enforced expression of TNK2 attenuated inhibitory effect of ZNF692 knockdown on P-MEK1/2 and P-ERK1/2 (- and + stand for categorical values absent and present, referring to gene knockdown or overexpression by means of the listed constructs) (J-L) Western blot and quantitative analyses of MEK1/2, P-MEK1/2, ERK1/2, and P-ERK1/2 for the indicated groups revealed that silencing TNK2 in osteosarcoma cells partially reversed promoting effect of ZNF692 overexpression on P-MEK1/2, P-ERK1/2. Student’s t-test and one-way ANOVA were performed to analyze differences between groups. All data are presented as means ± standard deviations (SD). *P < 0.05, **P < 0.01, ***P < 0.001