Fig. 4

ZNF692 positively regulates MEK/ERK signaling to promote cell proliferation, migration, and invasion. (A) Volcano plot illustrating the ZNF692-associated genes based on Pearson correlation analysis. (B) KEGG analysis revealed that ZNF692-associated genes were mainly enriched in MAPK signaling pathway. (C-D) Western blot analysis of MEK1/2, P-MEK1/2, ERK1/2, and P-ERK1/2 in osteosarcoma cells stably knocking down ZNF692, and quantitative analysis. (E-F) Western blot analysis of MEK1/2, P-MEK1/2, ERK1/2, and P-ERK1/2 in osteosarcoma cells stably overexpressing ZNF692, and quantitative analysis. (G) Application of U0126 (an inhibitor of MEK/ERK signaling) partially counteracted the enhanced cell proliferation generated by overexpression of ZNF692. (H) Application of U0126 partially counteracted the enhanced cell migration generated by overexpression of ZNF692. (I-J) Application of U0126 partially counteracted the enhanced cell invasion generated by overexpression of ZNF692. Student’s t-test and one-way ANOVA were performed to analyze differences between groups. All data are presented as means ± standard deviations (SD). *P < 0.05, **P < 0.01, ***P < 0.001