Fig. 4

MENA knockdown confers in vivo resistance against tamoxifen in the HR.⁺ breast cancer cells. A The in vivo study design of the xenograft tumor growth experiment in nude mice. The tumor-bearing mice were sacrificed on day 43. B Representative photographs of the xenograft tumor-bearing mice belonging to the MCF7-shNC, MCF7-shNC + TAM, MCF7-shMENA, and MCF7-shMENA + TAM groups. C Representative images of the xenograft tumors isolated from the MCF7-shNC, MCF7-shNC + TAM, MCF7-shMENA, and MCF7-shMENA + TAM groups of mice at the final time point. D The growth curve of the nude mice belonging to the MCF7-shNC, MCF7-shNC + TAM, MCF7-shMENA, and MCF7-shMENA + TAM groups based on the tumor sizes between days 0–16 after injection of the tumor cells and treatment with or without tamoxifen. E, F Histograms show the E tumor volumes and F and tumor weights of the MCF7-shNC, MCF7-shNC + TAM, MCF7-shMENA, and MCF7-shMENA + TAM groups of mice at the end-point. G The NENA protein level in MCF7-shNC, MCF7-shNC + TAM, MCF7-shMENA, and MCF7-shMENA + TAM groups detected by IHC. Bar = 100 μm. **p < 0.01; ***p < 0.001; n = 5