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Fig. 3 | Biology Direct

Fig. 3

From: Mammalian enabled protein enhances tamoxifen sensitivity of the hormone receptor-positive breast cancer patients by suppressing the AKT signaling pathway

Fig. 3

MENA knockdown increases invasion, migration, and tamoxifen resistance of the HR.+ BC cells. A Western blot analysis results show MENA protein expression levels in the shNC- and shMENA-transfected MCF7 and T47D cells. B-C RT-qPCR analysis results show the relative MENA mRNA levels in the shNC- and shMENA-transfected MCF7 and T47D cells. D-E CCK8 assay results show the viability status of the shNC- and shMENA-transfected MCF7 and T47D cells treated with or without 5 μm 4-OHT. F, G Representative images and quantitative analysis of the colony formation assay results show the total number of colonies formed by the shNC- and shMENA-transfected MCF7 and T47D cells treated with or without 5 μm 4-OHT. H-I Representative images and quantitative analysis of the transwell migration and invasion assays show the migration and invasion abilities of the shNC- and shMENA-transfected MCF7 and T47D cells treated with or without 5 μm 4-OHT. J Western blot analysis results show E-cadherin and Vimentin protein expression levels in the shNC- and shMENA-transfected MCF7 and T47D cells.*p < 0.05; **p < 0.01; ***p < 0.001; n = 3

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