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Fig. 2 | Biology Direct

Fig. 2

From: Mammalian enabled protein enhances tamoxifen sensitivity of the hormone receptor-positive breast cancer patients by suppressing the AKT signaling pathway

Fig. 2

MENA is downregulated in the tamoxifen-resistant HR.+ BRCA cells. A-B CCK-8 assay results show the viability status of MCF7 and T47D cells treated with different concentrations of 4-OHT (0–20 µM) for 4 days. The viability of 4-OHT-treated cells was normalized to the control cells. C-D CCK-8 assay results show the viability status of MCF7, MCF7-TR, T47D, and T47D-TR cells cultured with 4-OHT for 4 days. Viability of the parental strains (MCF-7 and T47D) cultured in 4-OHT-free media was set as 1. E Western blot analysis of MENA protein levels in the MCF7, MCF7-TR, T47D, and T47D-TR cells. Β-actin was used as a loading control. The left panel shows the representative image of the western blot. The right panel shows the relative MENA protein expression levels calculated for the 4 cell lines from the blot. The relative expression of MENA to β-actin in the parental strains (MCF7 or T47D) was set to 1.0. F-G RT-qPCR analysis results show the relative MENA mRNA levels in the T47D, T47D-TR, MCF7 and MCF7-TR cells. The relative MENA mRNA levels in the parental strains (MCF-7 or T47D) were set to 1.0. *p < 0.05, **p < 0.01, ***p < 0.001

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