Fig. 1

L. reuteri inhibits hepatic I/R-induced tissue damage, apoptosis, and inflammation. (A) Serum ALT, AST, and MPO activity levels were measured by biochemical kits. (B) The degree of hepatic tissue damage was observed by H&E staining, and Suzuki scores were obtained. Scale bar: 25 μm (400×). (C) The level of cell apoptosis in hepatic tissue was assessed by TUNEL fluorescence staining. Scale bar: 25 μm (400×). (D) The expression of Bcl2, Bax, and cleaved-Caspase3 was detected by western blot. (E) The levels of p-p65/p65, p-IκB/IκB, p-p38/p38, p-JNK/JNK, and p-ERK/ERK were detected by western blot. (F) Concentrations of IL-1β, IL-6, TNF-α, and IL-10 were assessed by ELISA. n = 10 mice/group. ^#P < 0.05 vs. Sham + vehicle; ^*P < 0.05 I/R-1 h + vehicle, I/R-6 h + vehicle, or I/R-24 h + vehicle; ^&P < 0.05 I/R-1 h + L. reuteri-low, I/R-6 h + L. reuteri-low, or I/R-24 h + L. reuteri-low