Fig. 6

A Through mass spectrometry (MS) analysis, proteins interacting with STAMBPL1 in HCCLM3 and Hep3B cells were identified, among which TRAF2 is one of the proteins obtained in the intersection. B Immunoprecipitation analysis identified an interaction between STAMBPL1 and TRAF2 in HCCLM3 cells. C Immunoprecipitation analysis identified an interaction between STAMBPL1 and TRAF2 in Hep3B cells. D Through GST-pull down analysis, a direct interaction between STAMBPL1 and TRAF2 was identified in HCCLM3 cells. E Cell Immunofluorescence analysis detected co-localization of STAMBPL1 and TRAF2 in the cytoplasm. F WB analysis revealed that in HCCLM3 cells, the expression level of TRAF2 decreases in the STAMBPL1 knockdown group and increases in the STAMBPL1 overexpression group. G WB analysis revealed that in Hep3B cells, the expression level of TRAF2 decreases in the STAMBPL1 knockdown group and increases in the STAMBPL1 overexpression group. H Heatmap and volcano plot illustrate that TRAF2 shows no significant differential expression in the obtained RNA-seq results. I After treating HCCLM3 cells with CHX (0.2 mg/ml), the TRAF2 protein levels were assessed over specified time points following the downregulation of STAMBPL1. J After treating Hep3B cells with CHX (0.2 mg/ml), the TRAF2 protein levels were assessed over specified time points following the downregulation of STAMBPL1. K After treatment with 20 μM MG132, the levels of TRAF2 were assessed in HCCLM3 cells following the downregulation of STAMBPL1. L After treatment with 20 μM MG132, the levels of TRAF2 were assessed in Hep3B cells following the downregulation of STAMBPL1