Fig. 5

A Heatmap illustrating the RNA-seq analysis results of sh-control and sh-STAMBPL1 group in stable transfected HCCLM3 cell line. B Volcano plot illustrating the DEGs in the sh-control and sh-STAMBPL1 groups of HCCLM3 cell line. C The KEGG enrichment analysis reveals that DEGs are primarily enriched in the WNT and NF-KB pathways in HCCLM3 cell. D Heatmap illustrating the RNA-seq analysis results of sh-control and sh-STAMBPL1 groups in stable transfected Hep3B cell line. E Volcano plot illustrating the DEGs in the sh-control and sh-STAMBPL1 groups of Hep3B cell. F The KEGG enrichment analysis reveals that DEGs are primarily enriched in the PI3K/AKT pathways in Hep3B cell. G Validation through Western blot of differential expression of key proteins in the WNT/PI3K/NF-kb signaling pathway between the sh-control and sh-STAMBPL1 group of HCCLM3 cell and Hep3B cell. H Validation through Western blot of differential expression of key proteins in the WNT/PI3K/NF-kb signaling pathway between the vector group and the STAMBPL1 over-expressed group of HCCLM3 and Hep3B cells. I Detection using cell immunofluorescence (ICC/IF) revealed that knocking down STAMBPL1 inhibited the nuclear translocation of the P65 protein in HCCLM3 cell. J Detection using cell immunofluorescence (ICC/IF) revealed that knocking down STAMBPL1 inhibited the nuclear translocation of the P65 protein in Hep3B cell