Fig. 3

A, B Analyzing the expression levels of STAMBPL1 in five different types of HCC cells through Western blot analysis. C Validating the knockdown efficiency of shRNA targeting STAMBPL1 in HCCLM3 cells using qRT-PCR. D Validating the knockdown efficiency of shRNA targeting STAMBPL1 in Hep3B cells using qRT-PCR. E Western blot validation of the knockdown efficiency of shRNA targeting STAMBPL1 in HCCLM3 cell. F Western blot validation of the knockdown efficiency of shRNA targeting STAMBPL1 in Hep3B cell. G CCK-8 experiment to assess the proliferation efficiency between the sh-control group and the sh-STAMBPL1 group in HCCLM3 and Hep3B cell. H The colony formation assay to examine the proliferation efficiency of the sh-control group and the sh-STAMBPL1 group HCCLM3 and Hep3B cell. I The EdU assay to assess the proliferation efficiency between sh-control group and the sh-STAMBPL1 group in HCCLM3 cell. J The EdU assay to assess the proliferation efficiency between sh-control group and the sh-STAMBPL1 group in Hep3B cell. K Performing Western blot analysis to identify the Cyclin D1, P21, PCNA, and Ki-67 expression in both the sh-control and sh-STAMBPL1 groups of HCCLM3 and Hep3B cells. L The transwell assay to evaluate the migration and invasion efficiency between the sh-control and sh-STAMBPL1 group of HCCLM3 cell. M The transwell assay to evaluate the migration and invasion efficiency between the sh-control and sh-STAMBPL1 group of Hep3B cell. N The cell scratch assay to assess the migration efficiency of the sh-control and sh-STAMBPL1 group in HCCLM3 cell. O The cell scratch assay to assess the migration efficiency of the sh-control and sh-STAMBPL1 group in Hep3B cell. P Western blot analysis to detect the protein expression levels of E-cadherin, N-cadherin, MMP3, Vimentin and Snail in the sh-control and sh-STAMBPL1 group of HCCLM3 cells. Q Conducting Western blot analysis to identify the protein expression levels of E-cadherin, N-cadherin, MMP3, Vimentin, and Snail in both the sh-control and sh-STAMBPL1 groups of Hep3B cells