Fig. 1

A Heatmap represents the results of RNA-seq on 3 pairs of HCC tissue and the corresponding adjacent non-cancerous tissue. B The volcano plot displays that 4972 genes are upregulated, and 4602 genes are downregulated in the RNA-seq results. C By intersecting the differential expressed genes (DEGs) from TCGA, the ubiquitinase list (DUBs), and DEGs of RNA-seq, we ultimately obtained 7 genes. D The bar chart displays the fold change of 7 genes (USP14, USP1, USP27X, SENP1, OTUD3, OTUB2, STAMBPL1) expression in HCC compared to the non-cancerous tissue. E By utilizing paired t-test analysis on gene expression data and corresponding patient information from the TCGA database, the high expression of STAMBPL1 was identified. F By utilizing independent samples t-test analysis on gene expression data and corresponding patient information from the TCGA database, the high expression of STAMBPL1 was identified. G Through the application of paired t-test analysis on gene expression data alongside corresponding patient information from the GSE46408 dataset, the high expression of STAMBPL1 was identified. H By utilizing independent samples t-test analysis on gene expression data and corresponding patient information from the GSE112790 dataset, the high expression of STAMBPL1 was identified. I By utilizing independent samples t-test analysis on gene expression data and corresponding patient information from the GSE121248 dataset, the high expression of STAMBPL1 was identified. J The expression of STAMBPL1 is significantly correlated with TP53 mutations. K The expression of STAMBPL1 is significantly correlated with the pathological stage of HCC patients. L The expression of STAMBPL1 is significantly correlated with the presence or absence of lymph node metastasis. M The STAMBPL1 expression shows a significant correlation with the pathological grade of patients with HCC. N The cBioPortal database indicates that mutations in STAMBPL1 predominantly include amplifications, deletions, and missense mutations. O The TIMER online database confirmed that STAMBPL1 is significantly overexpressed in pan-cancer, including in HCC. P Further confirmation was made through the use of the TCGA database that STAMBPL1 is significantly overexpressed in pan-cancer, including in HCC