Fig. 2From: Effect of glycosylation on the affinity of the MTB protein Ag85B for specific antibodies: towards the design of a dual-acting vaccine against tuberculosis(a) Chemical structure of the different glycans activated with IME linker. (b) Chemical glycosylation of proteins. Reactions were performed in 100 mM sodium tetraborate pH 9.5 at 25 °C for 24 h; glycosidic reagent/protein molar ratio: 250:1 for Ara3Man-rAg85B-K30R/K282R, and 200:1 for the others; protein concentration: 2 mg/mL. In rAg85B and rAg85-K30R/K282R glycosylation, 1mM benzamide hydrochloride was added in the reaction mixture to avoid potential proteolysis [33]. Protein graphical representation depicts Ag85B protein and was taken from the Protein data Bank (PDB DOI: https://doi.org/10.2210/pdb1f0p/pdb)Back to article page