Fig. 3
From: RPL35A promotes the progression of cholangiocarcinoma by mediating HSPA8 ubiquitination
RPL35A knockdown promoted the ubiquitination of HSPA8 protein (A) Base peak graph of IP-MS detection results. (B) Coomassie brilliant blue staining was employed to detect protein expression in the RPL35A overexpression group and the control group and the protein bands was identified by mass spectrometry. (C) The protein-protein interaction network diagram showed the results of predicting proteins that might interact with RPL35A using STRING online tool. (D) The HSPA8 levels in CCA and normal samples was predicted by GEPIA online tool. (E) The interaction between RPL35A and HSPA8 was determined by Co-IP. (F) HCCC-9810 and HUCCT1 cells in RPL35A knockdown group and control group were treated with CHX, and the HSPA8 protein levels were detected by western blot at 0, 2, 4, and 8 h, respectively. (G) HSPA8 protein levels in HCCC-9810 and HUCCT1 cells treated with/without MG-132 in RPL35A knockdown group and control group were detected by western blot. (H) Co-IP was used to detect the ubiquitin level of HSPA8 protein after MG-132 treatment of HCCC-9810 and HUCCT1 cells. *P < 0.05