Fig. 4

TM4SF1 is required for PLAU-induced aggressive phenotype. (A) Western blot analysis of TM4SF1 protein levels in A549 and H1299 cells transfected with shRNAs targeting TM4SF1. (B) Assessment of the proliferation of NSCLC cells transfected with indicated plasmids by direct counting. ^*P < 0.05. (C) NSCLC cell proliferation as measured by EdU assay. ^*P < 0.05. (D) Colony formation assay. ^*P < 0.05. (E) Apoptosis analysis by annexin-V and propidium iodide staining. NSCLC cells transfected with indicated plasmids were cultured in the presence or absence of serum for 48 h before apoptosis assay. ^*P < 0.05. (F) NSCLC cells transfeced with indicated plasmids were treated with different concentrations of cisplatin for 72 h before viability assessment by the MTT method