Fig. 7

miR-24-3p binds to and negatively modulates OGT expression. a Venn diagram depicting the intersection of predicted miRNAs targeting OGT. b The relationship between OGT and miRNAs in NSCLC patients was examined via LinkedOmics analysis. c qRT-PCR was employed to detect OGT mRNA expression. d Western blotting was utilized to analyze OGT protein expression. e TargetScan was used to predict miR-24-3p binding sites in the 3′-UTR of OGT mRNA. f Direct interaction between miR-24-3p and OGT was confirmed by the dual-luciferase reporter assay. g The assessment of cell proliferation was accomplished via the CCK-8 assay. h The monitoring of cell colony-forming ability was executed using the colony formation assay. i The measurement of cell migration and invasion was carried out utilizing the Transwell assay. ^*P < 0.05, ^**P < 0.01, and ^#P > 0.05