Fig. 6
From: The oncogenic role and regulatory mechanism of PGK1 in human non-small cell lung cancer
O-GlcNAcylation regulates PGK1 function. a The detection of O-GlcNAcylation levels was performed through immunoprecipitation. b-d The impact of PGK1 mutation on NSLCL cell growth, migration, and invasion was assessed using the CCK-8, colony formation, and Transwell assays, respectively. e An animal experiment was conducted to evaluate the effect of PGK1 mutation on tumor growth. f Immunohistochemical staining of Ki-67 in xenograft tumors from the nude mice. g-i Cell growth, migration, and invasion in different groups were separately measured by the CCK-8, colony formation, and Transwell assays. MUT, pcDNA3.1( +)-PGK1 wild-type plasmid; MUT, pcDNA3.1( +)- PGK1 mutant plasmid. *P < 0.05,**P < 0.01