Fig. 5
From: DTX2 promotes glioma development via regulation of HLTF
HLTF inhibits cell proliferation and migration of glioma cells. A The knockdown efficiency of HLTF in glioma cells was confirmed using western blotting. B The role of HLTF knock down in glioma cell proliferation was detected using the CCK-8 assay. (**P < 0.001). C Numbers of colonies formed by U87 and U251 cells transfected with shHLTF #1 and shHLTF #2. (***P < 0.001). D The effects of HLTF knock down in glioma cell migration were investigated using a Transwell assay. (***P < 0.001). E The efficiency of HLTF overexpression using HLTF overexpression plasmids in glioma cells was confirmed using western blotting. F The role of HLTF overexpression in glioma cell proliferation was detected using the CCK-8 assay. (***P < 0.001). G Numbers of colonies formed by U87 and U251 cells transfected with HLTF overexpression plasmids. (***P < 0.001). H The effects of HLTF overexpression in glioma cell migration were investigated using a Transwell assay. (H) The effects of HLTF in glioma cell migration were investigated using the wound healing assay