Fig. 4

Upregulation of LRIG1 suppresses the progression of bladder carcinoma. A The mRNA level of LRIG1 in 90 pairs of bladder carcinoma tissues and adjacent noncancerous specimens was determined using the qRT-PCR assay. B The protein level of LRIG1 in 3 pairs of bladder carcinoma tissues and adjacent noncancerous specimens was determined using the Western blot assay. C The co-localization of circLRIG1 and LRIG1 was observed in UMUC3 and T24 cells (magnification, × 200, Scale bar, 50 μm) by FISH assay. D The mRNA and protein levels of LRIG1 in bladder carcinoma cell lines (UMUC3 and T24) after transfection with vector-circ or oe-circLRIG1 plasmids were determined using the qRT-PCR and Western blot assay. E The correlation between the expression of circLRIG1 and LRIG1 in bladder carcinoma tissues was analyzed using Pearson’s correlation coefficient. F The protein levels of LRIG1 in bladder carcinoma cell lines (UMUC3 and T24) after transfection with OE-vector or OE-LRIG1 plasmids were determined using the Western blot assay. G The cell viability was determined using the CCK-8 assay. H The number of cell colonies was determined using the colony formation assay. I The number of apoptotic cells was determined using the flow cytometry analysis. The experiment was performed in triplicate. “**” indicates P < 0.01