Fig. 6

Inhibition of mitolysosome formation by oxidative stress induces microglial senescence. (A) Representative images of BV2 cells stained with SA-β-Gal treated with or without 150 µM H₂O₂ for 72 h. Scale Bar, 50 μm. (C) Relative mRNA levels of p16, p21 and p53 in BV2 cells treated with or without 150 µM H₂O₂ for 72 h (n = 3–4). (D) Relative mRNA level of p53 in BV2 cells treated with or without 150 µM H₂O₂ in the absence or presence of UA as indicated for 72 h (n = 4). (E) Representative images of BV2 cells stained with SA-β-Gal treated with or without 150 µM H₂O₂ in the absence or presence of UA as indicated for 72 h. Scale Bar, 50 μm. (B, F) Graphs represent percent of SA-β-Gal positive cells (green staining) per imaging field. (B) n = 6 imaging fields and (F) n = 5–6 imaging fields. Data are means ± SEM; ** p < 0.01 and *** p < 0.001 versus no treatment (control) group, ^##p < 0.01 and ^###p < 0.001 versus H₂O₂ alone treatment group; Two-tailed unpaired t test (B and C) or one-way ANOVA followed by Tukey’s multiple comparisons test (D and F)